| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-WIPI1 (350-400 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | WIPI1 |
| Gene ID: | 55062 |
| Uniprot ID: | WIPI1_HUMAN |
| Immunogen Region: | 350-400 aa |
| Specificity: | Specific for WIPI1 |
| Immunogen: | A synthetic peptide from aa region 350-400 of human WIPI1 conjugated to blue carrier protein was used as the antigen. The peptide shares 91% identity with mouse sequence. |
| Function | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Together with WDR45/WIPI4, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Also plays a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. |
| Protein Name | Wd Repeat Domain Phosphoinositide-Interacting Protein 1 Wipi-1 Atg18 Protein Homolog Wd40 Repeat Protein Interacting With Phosphoinositides Of 49 Kda Wipi 49 Kda |
| Database Links | Reactome: R-HSA-1632852 Reactome: R-HSA-381038 |
| Cellular Localisation | Golgi Apparatus Trans-Golgi Network Endosome Cytoplasmic Vesicle Clathrin-Coated Vesicle Preautophagosomal Structure Membrane Peripheral Membrane Protein Cytoplasm Cytoskeleton Trans Elements Of The Golgi And Peripheral Endosomes Dynamically Cycles Through These Compartments And Is Susceptible To Conditions That Modulate Membrane Flux Enriched In Clathrin-Coated Vesicles Upon Starvation-Induced Autophagy Accumulates At Subcellular Structures In The Cytoplasm: Enlarged Vesicular And Lasso-Like Structures And Large Cup-Shaped Structures Predominantly Around The Nucleus Recruitment To Autophagic Membranes Is Controlled By Mtmr14 Labile Microtubules Specifically Recruit Markers Of Autophagosome Formation Like Wipi1 Whereas Mature Autophagosomes May Bind To Stable Microtubules |
| Alternative Antibody Names | Anti-Wd Repeat Domain Phosphoinositide-Interacting Protein 1 antibody Anti-Wipi-1 antibody Anti-Atg18 Protein Homolog antibody Anti-Wd40 Repeat Protein Interacting With Phosphoinositides Of 49 Kda antibody Anti-Wipi 49 Kda antibody Anti-WIPI1 antibody Anti-WIPI49 antibody |
Information sourced from Uniprot.org